A Pilot Study Assessing a Concentration of 100 mg/L Alizarin Complexone (ALC) to Mark Calcified Structures in Hypophthalmichthys molitrix

The effectiveness of chemical compounds for marking hard tissues in juvenile silver carp (Hypophthalmichthys molitrix) is not well known. We analyzed the use alizarin complexone (ALC) as a fluorescent marker to mark various structures carp. Experimental fish (~2 months old) were randomly assigned either control or groups, which immersed 0 100 mg/L ALC solutions, respectively, 2 days. otoliths, fin rays, and scales then sampled, visualized using fluorescence microscopy, evaluated after 10 treatment was effective certain color affected by light source. There no obvious differences efficiency rays from pectoral, dorsal, ventral, anal, caudal fins, but lapilli lateral line marked most effectively sampled otolith scale types, respectively. Our findings indicate that immersion ray sampling can be used non-lethal evaluation